Journal: NPJ Vaccines

Article Title: A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19

doi: 10.1038/s41541-021-00352-1

Figure Lengend Snippet: a Left: genome structure of the VSV vector expressing the membrane-anchored SARS-CoV2 S1 domain. The S1 domain of SARS-CoV2 spike protein (aa 1–681) was joined to the C-terminal 70 amino acids of the VSV glycoprotein. The fusion construct was inserted between the glycoprotein and polymerase genes of VSV. The S1 domain is shown in red and the VSV G tail in green. The transmembrane domain of the VSV glycoprotein is indicated by a yellow box. The amino acids at the junction between S1 and VSV glycoprotein are highlighted. Right: schematic representation of the vaccine construct that shows the two transmembrane proteins anchored in the membrane. b Immunofluorescence staining of Vero E6 cells infected with ConVac and a control virus expressing Hendra virus G (VSV-HeVG). The cells were fixed and permeabilized 10 h after infection and stained with fluorescently labeled monoclonal antibody CR3022 against the S1 domain (shown in red) and two monoclonal antibodies against the VSV glycoprotein (shown in green). c Western blot analysis of BSR cells infected with Convac and a control VSV virus expressing GFP. Protein lysates were resolved on 4–20% polyacrylamide-gradient gels and transferred to nitrocellulose membranes. The membranes were probed with polyclonal antiserum against the S1 domain (upper panel), monoclonal antibodies against the VSV glycoprotein (middle panel), and a monoclonal antibody against the VSV matrix protein (lower panel). VSV glycoprotein expression was significantly reduced in cells infected with ConVac whereas matrix protein expression was only modestly affected. d Viral growth curve on Vero cells. The cells were infected at an MOI of 0.05 PFU with ConVac, or VSV-expressing GFP or another control virus expressing Hendra virus glycoprotein. Supernatants were collected 12, 24, and 36 h post infection and titrated on Vero E6 cells. Data represent mean ± SD.

Article Snippet: The proteins were transferred to nitrocellulose membranes and probed with polyclonal rabbit serum against SARS-CoV S1 domain (Thermofisher, Cat No. PA5-81798 and PA5-81795), monoclonal antibodies I1 (8G5F11) and I14 (IE9F) against the VSV glycoprotein, and monoclonal antibody 23H12 against VSV matrix protein provided by Douglas Lyles (Wake Forest University).

Techniques: Plasmid Preparation, Expressing, Membrane, Construct, Immunofluorescence, Staining, Infection, Control, Virus, Labeling, Bioprocessing, Western Blot

Journal: Cell host & microbe

Article Title: Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways During HIV Infection

doi: 10.1016/j.chom.2018.01.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TaqMan ISG15 assay , ThermoFisher , Cat# Hs01921425_s1.

Techniques: Virus, Recombinant, Saline, High Molecular Weight, Electron Microscopy, SYBR Green Assay, Staining, Library Quantification, Next-Generation Sequencing, Fractionation, Plasmid Preparation, DNA Purification, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Luciferase, Infection, Sequencing, Cloning, Control, shRNA, Software, Flow Cytometry, Gene Expression, Microarray

Journal: Cell Chemical Biology

Article Title: Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter

doi: 10.1016/j.chembiol.2021.07.016

Figure Lengend Snippet:

Article Snippet: PPIA TaqMan® Gene Expression Assay , ThermoFisher Scientific , Cat# Hs04194521_s1.

Techniques: Virus, Subcloning, Recombinant, Saline, Reverse Transcription, Plasmid Preparation, Gene Expression, Negative Control, Positive Control, Software, Drug discovery, High Content Screening, Real-time Polymerase Chain Reaction